DESCRIPTION
IPTG (Isopropyl-¥â-D-thio-galactopyranoside) is an inducer of ¥â-galactosidase activity in bacteria. It is suitable for use with X-Gal to detect lac gene activity in cloning procedures. This product is free of dioxane contamination.
STOCK SOLUTION
Dissolve 2 g of IPTG in 8 ml of distilled H2O and adjust the volume to 10 ml with distilled H2O. Sterilize by filtration through 0.22 ¥ìm filter. Dispense into 1ml aliquots and store at 4 or -20¡É.
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MOLECULAR WEIGHT: 238.3
MELTING POINT: 107-114¡É
CAUTION
Suspected carcinogen. Avoid breathing dust. Avoid contact with skin and clothing. Wash thoroughly with soap and water after handling.
STORAGE CONDITIONS
Store at 4 or -20¡É. Shelf storage is O.K. for several weeks. Keep dry, protect from light.
REFERENCE 1. Sambrook, J., et al., Mlecular cloning: A Laboratory Manual 2nd ed., Cold Spring Harbor Laboratory Press (Cold Spring Harbor, NY: 1989), 1.8-1.9, 1.86 |