DESCRIPTION
AMV (Avian Myeloblastosis Virus) Reverse Transcriptase is an RNA-dependent DNA polymerase. This enzyme catalyses the polymerization of DNA using single-stranded DNA or RNA as a template in the presence of a primer. It also has a 3' ¡æ 5' RNase H activity that degrades the RNA portion of RNA-DNA hybrids.
SOURCE
Purified from Avian Myeloblastosis Virus.
APPLICATIONS
¡Ü cDNA synthesis.
¡Ü DNA sequencing by dideoxy method.
¡Ü RNA sequencing.
¡Ü 3'-end labeling of DNA fragments.
UNIT DEFINITION
One unit is defined as the amount of enzyme required to catalyze the incorporation of 1 nmol of dTTP into acid-insoluble form in 10 minutes at 37¡É.
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STANDARD UNIT ASSAY CONDITIONS
50 mM Tris-Cl (pH 8.3), 40 mM KCl, 7 mM MgCl2, 1 mM DTT, 0.1 §·/§¢ BSA, 0.5 mM radiolabeled dTTP, and 35 §¶/§¢ rA400:dT50.
CONCENTRATION: 5-10 units/§¡, 20-40 units/§¡ (High concentration product)
5X REACTION BUFFER
250 mM Tris-Cl (pH 8.3), 250 mM KCl, 50 mM MgCl2, 2.5 mM spermidine, 50 mM DTT.
STORAGE CONDITIONS
0.1 M KPO4 (pH 7.2), 0.2% Triton X-100, 2 mM DTT, and 50% glycerol. Store at -70¡É.¡¡
REFERENCES
1. Okayama, H. et al. (1987) Methods Enzymol. 154, 3.
2. Pfeffer, D. and Mierendorf, R. (1987) Methods Enzymol. 152, 563.
3. Houts, G.E. et al. (1979) J. Virol. 29, 517.
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