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DNA Polymerase I, Klenow Fragment / 150U / Cat.#3004
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  DNA Polymerase I

  Large (Klenow) Fragment

    

#3004

 

150 units

#3004L

 

750 units

 

 

DESCRIPTION 

Klenow Fragment is a proteolytic product of intact DNA polymerase I which retains 5'¡æ3' DNA polymerase and 3'¡æ5' exonuclease activity but lacks 5'¡æ3' exonuclease activity. 

 

 

SOURCE 

Recombinant E. coli strain. 

 

 

APPLICATIONS 

¡Ü DNA sequencing by the Sanger dideoxy method. 

¡Ü Filling-in and/or labeling of recessed  3'-ends. 

¡Ü Second strand cDNA synthesis. 

¡Ü End-labeling of DNA molecules with  protruding 3' 

tails. 

¡Ü Synthesis of dsDNA from ssDNA templates 

  during in vitro mutagenesis. 

 

 

UNIT DEFINITION 

One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTPs into acid-insoluble form in 30 minutes at 37¡É. 

 

 

 

STANDARD UNIT ASSAY CONDITIONS 

67mM KPO4 (pH7.5), 6.7mM MgCl2, 1mM DTT, 133¥ìM activated calf thymus DNA and 33¥ìM dATP, dCTP, dGTP and [3H]-dTTP. 

 

 

CONCENTRATION: 5-10 units/¥ìl. 

 

 

10X REACTION BUFFER 

100mM Tris-HCl (pH7.5@37¡É), 50mM MgCl2, 7.5mM DTT. dNTPs not included. 

 

 

STORAGE CONDITIONS 

50mM Tris-HCl (pH7.5), 1mM DTT, 0.1mM EDTA and 50% glycerol.  Store at -20¡É. 

 

 

REFERENCES 

1. Sanger, F. et al. (1977) Proc. Natl. Acad. Sci. USA 74, 5463. 

2. Joyce, D.M. and Grindley, N.D.F. (1983) Proc. Natl. Acad. Sci. USA 80, 1830. 

3. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, second ed., Cold Spring Harbor Laboratory, Cold Spring Harbor. 

4. Gubler, U. (1987) Methods Enzymol. 152, 330. 

 

 

 


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