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 DESCRIPTION  
 Mung Bean Nuclease degrades single-stranded nucleic acids to yield 5'-phosphoryl mono- or oligonucleotides.  Double-stranded DNA, RNA and DNA:RNA hybrids are relatively resistant to the enzyme.  If very large amount of enzyme are used it also degrades double-stranded DNA from both ends.  Unlike S1 nuclease, this enzyme will not cleave the DNA strand opposite a nick in a duplex.  
   
   
 SOURCE  
 Mung bean sprouts.  
   
   
 APPLICATIONS  
 ¡Ü Removal of 3' and 5' extensions from DNA or RNA  
 termini.  
 ¡Ü Transcript mapping.  
 ¡Ü Opening up hairpin loops formed during synthesis of  
 double-stranded cDNA.  
 ¡Ü Excision of gene coding sequences from genomic  
   DNA.  
   
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 UNIT DEFINITION  
 One unit is defined as the amount of enzyme required to produce 1¥ìg of acid-soluble nucleotides 1 minutes at 37¡É.  
   
   
 STANDARD UNIT ASSAY CONDITIONS  
 30mM sodium acetate (pH4.6), 50mM NaCl, 1mM ZnCl2, 0.5mg/ml denatured calf thymus DNA and 5%(v/v) glycerol.  
   
   
 CONCENTRATION: 50-100 units/ul.  
   
   
 10X REACTION BUFFER  
 300mM sodium acetate(pH5.0), 500mM NaCl, 10mM ZnCl2.  
   
   
 STORAGE CONDITIONS  
 10mM sodium acetate (pH5.0), 0.1mM zinc acetate, 1mM cysteine, 0.001% Triton X-100, and 50% glycerol.  Store at -20¡É.  
   
   
 REFERENCES  
 1. Kowalski, D. et al. (1976) Biochemistry 15, 4457.  
 2. Green, M.R. and Roeder, R.G. (1980) Cell 22, 231.  
 3. Gubler, U. (1987) Methods Enzymol. 152, 330.  
   
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