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SP Sepharose FF, Pre-packed column
- resin : SP Sepharose FF, GE Healthcare
- packing : 10ml, 1set (cat.# DSP-10-02)
- Accessories included : 1/16" male union, 1ea.
6mm male union, 1ea.
2Φx0.5tx1m PTFE tubing(with 6mm union), 1set
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BPXTM Ds10-SP : Cat.#DSP-10-02
BPXTM Ds10-SP are prepacked SP Sepharose Fast Flow columns for strong cation exchanger, which are used for small-scale protein purification as well as for screening of binding and elutions conditions.
- Convenient and affordable design for fast, easy ion exchange separations
- High flow rates and high capacities.
- Easy & predictable scale-up
- Compatible design for use with syringes, single laboratory pump, or chromatography system
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Column |
BPXTM DSC-102 |
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Column Volume
Bed Volume |
10 ml
10ml (15Φ x 57 mm) |
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Column Dimensions |
17Φ x 102 mm |
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Flow rate |
1 ml/min - 9 ml/min |
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Flow Velocity |
> 600 cm/hr |
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Pressure Max. |
1.5 bar [0.15 MPa] (22 psi) |
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Material |
Polypropylene (PP) |
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Bed Support & Filter |
Polypropylene (PP) & STS316L Sintered wire mesh filter |
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Media |
GEH Cat.#17-0510 / Q Sepharose FF |
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Ligand |
Sulphopropyl |
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Average Particle Size |
90 µm |
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Ion Exchanger Type |
Strong cation exchanger |
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Matrix |
6% cross-linked agarose |
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Particle Size |
45 µm-165 µm |
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Ionic Capacity |
0.18-0.25 mmol Cl-/ml medium |
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pH stability |
Working, 4-13
Cleaning, 3-14 |
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Flow Specification |
400-700 cm/h, 100 kPa, BPX 50/25 column, bed height 15 cm |
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Exclusion Limit [Mr] [Globular Proteins] |
4 x 106 |
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Storage Conditions |
4 to 30°C, 20% Ethanol |
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Estimated Shelflife |
5 years from Manufacture Date |
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Chemical Stability |
Stable to all commonly used aqueous buffers:1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol1) |
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l CIP : 1 N NaOH and acetic acid should be used only for cleaning purposes. |
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