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Native Protein A Pre-packed column
- resin : nProtein A Sepharose 4 FF, GE Healthcare
- packing : 10ml, 1set (cat.# DSP-10-07n)
- Accessories included : 1/16" male union, 1ea.
6mm male union, 1ea.
2Φx0.5tx1m PTFE tubing(with 6mm union), 1set
Plug valve, 1ea.; 보너스이벤트
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BPXTM Ds10-nProtein A : Cat.#DSP-10-07n
BPXTM Ds10-nProtein A are prepacked native Protein A coupled to Sepharose 4 Fast Flow for the purification of monoclonal and polyclonal antibodies at both laboratory and process scale.
nProtein A Sepharose 4 Fast Flow is native protein A coupled to the well established Sepharose 4 Fast Flow base matrix. The native protein A ligand is produced by fermenting a selected strain of Staphylococcus aureus. The purified protein is coupled to the cross-linked 4% agarose base matrix by the cyanogen bromide technique, giving a highly stable medium with minimal non-specific adsorption. nProtein A Sepharose 4 Fast Flow is manufactured without using animal-derived components.
nProtein A Sepharose 4 Fast Flow has nearly twice the total IgG binding capacity of Protein A Sepharose CL-4B, and is suitable for recovery and purification of monoclonal antibodies from cell culture at both laboratory and process scale. nProtein A Sepharose 4 Fast Flow was developed and tested in co-operation with leading manufacturers of purified monoclonal antibody products, and is used in routine commercial production.
- Quick, convenient preparative purification of monoclonal antibodies.
- Free from animal-derived components.
- Well-established protein A-based medium used in many approved MAb processes.
- Recombinant protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
- High flow rates and high capacities
- Easy & predictable scale-up
- Compatible design for use with syringes, single laboratory pump, or chromatography system
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Column |
BPXTM DSC-102 |
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Column Volume
Bed Volume |
10 ml
10 ml (15Φ x 57 mm) |
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Column Dimensions |
17Φ x 102 mm |
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Flow rate |
1 ml/min - 6 ml/min |
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Flow Velocity |
> 600 cm/hr |
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Pressure Max. |
1.5 bar [0.15 MPa] (22 psi) |
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Material |
Polypropylene (PP) |
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Bed Support & Filter |
Polypropylene (PP) & STS316L Sintered wire mesh filter |
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Media
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GE Healthcare Cat.# 17-5280-01 / nProtein A 4 FF
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Ligand |
native Staphylococcal Protein A (S. aureus) |
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Average Particle Size |
90 µm |
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Type |
native Protein A Affinity |
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Matrix |
4% cross-linked agarose |
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Ligand conc. |
6 mg Protein A / ml medium |
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Ligand coupling method |
Cyanogen bromide activation |
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Binding Capacity |
> 30 mg/ml hIgG |
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pH stability |
Working, 3-9
Cleaning, 2-10 |
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Flow Specification |
< 300 cm/h, 100 kPa, BPX 50/25 column, bed height 15 cm |
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Exclusion Limit [Mr] [Globular Proteins] |
4 x 106 |
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Storage Conditions |
4 to 30°C, 20% Ethanol |
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Estimated Shelflife |
3 years from Manufacture Date |
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Chemical Stability |
Stable to all commonly used aqueous buffers and 20% ethanol1) |
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l CIP : 0.1 N NaOH cleaning is not applicable. 0.1M Glycine(pH3.0) is generally safe for CIP. | |
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