Phenyl Sepharose 6 FF(high), Pre-packed column
- resin : Phenyl Sepharose 6 FF (high substitution) GEH Cat.17-0973-01
- packing : 5ml, 2set (cat.# DSP-05-032)
5set(cat.# DSP-05-035)
- Accessories included : 1/16" male union, 1ea./set
6mm male union, 1ea./set
2Φx0.5tx1m PTFE tubing(with 6mm union), 1set/set
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BPXTM Ds5-Phenyl : Cat.#DSP-05-03
BPXTM Ds5-Phenyl are prepacked Phenyl Sepharose 6 Fast Flow(high substitution) columns for hydrophobic interaction chromatography (HIC). The columns are used for small-scale protein purification as well as for screening of binding and elutions conditions.
- Convenient and easy small-scale protein purifications.
- Phenyl Sepharose 6 Fast Flow is a well-established, standard aromatic HIC medium for capture and intermediate purification requiring low to medium hydrophobicity.
- Flexible design for use with syringes, single laboratory pump, or chromatography system such as ÄKTA design.
- High flow rates and high capacities.
- Easy & predictable scale-up
- Compatible design for use with syringes, single laboratory pump, or chromatography system
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Column |
BPXTM DSC-101 |
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Column Volume
Bed Volume |
5 ml
5ml (12Φ x 45 mm) |
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Column Dimensions |
14Φ x 85 mm |
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Flow rate |
1 ml/min - 5 ml/min |
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Flow Velocity |
> 600 cm/hr |
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Pressure Max. |
1.5 bar [0.15 MPa] (22 psi) |
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Material |
Polypropylene (PP) |
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Bed Support & Filter |
Polypropylene (PP) & STS316L Sintered wire mesh filter |
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Media |
GEH Cat.#17-0973-01 / Phenyl Sepharose 6 FF (High substitution) |
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Ligand
Spacer arm |
Phenyl
2-hydroxypropyl |
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Average Particle Size |
90 µm |
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Type |
Hydrophobic Interaction Chromatography |
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Matrix |
6% cross-linked agarose |
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Particle Size |
45 µm-165 µm |
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Degree of substitution |
approx. 40 μmol /ml medium |
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pH stability |
Working, 3-13
Cleaning, 2-14 |
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Flow Specification |
200-400 cm/h, 100 kPa, BPX 50/50 column, bed height 25 cm |
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Storage Conditions |
4 to 30°C, 20% Ethanol |
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Estimated Shelflife |
5 years from Manufacture Date |
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Chemical Stability |
Stable in commonly used aqueous buffers - 1 M NaOH, 3 M Ammonium sulphate, 30% isopropanol, 70% ethanol, 10% ethylene glycol, 0.5% SDS, 6 M guanidine-hydrochloride, 8 M Urea |
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l CIP : 1 N NaOH should be used only for cleaning purposes. |
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