Native Protein A Pre-packed column
- resin : nProtein A Sepharose 4 FF, GE Healthcare
- packing : 5ml, 1set (cat.# DSP-05-07n)
- Accessories included : 1/16" male union, 1ea.
6mm male union, 1ea.
2Φx0.5tx1m PTFE tubing(with 6mm union), 1set
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BPXTM Ds5-nProtein A are prepacked native Protein A coupled to Sepharose 4 Fast Flow for the purification of monoclonal and polyclonal antibodies at both laboratory and process scale.
nProtein A Sepharose 4 Fast Flow is native protein A coupled to the well established Sepharose 4 Fast Flow base matrix. The native protein A ligand is produced by fermenting a selected strain of Staphylococcus aureus. The purified protein is coupled to the cross-linked 4% agarose base matrix by the cyanogen bromide technique, giving a highly stable medium with minimal non-specific adsorption. nProtein A Sepharose 4 Fast Flow is manufactured without using animal-derived components.
nProtein A Sepharose 4 Fast Flow has nearly twice the total IgG binding capacity of Protein A Sepharose CL-4B, and is suitable for recovery and purification of monoclonal antibodies from cell culture at both laboratory and process scale. nProtein A Sepharose 4 Fast Flow was developed and tested in co-operation with leading manufacturers of purified monoclonal antibody products, and is used in routine commercial production.
- Quick, convenient preparative purification of monoclonal antibodies.
- Free from animal-derived components.
- Well-established protein A-based medium used in many approved MAb processes.
- Recombinant protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
- High flow rates and high capacities
- Easy & predictable scale-up
- Compatible design for use with syringes, single laboratory pump, or chromatography system
|
Column |
BPXTM DSC-101 |
|
Column Volume
Bed Volume |
5 ml
5 ml (12Φ x 45 mm) |
|
Column Dimensions |
14Φ x 85 mm |
|
Flow rate |
1 ml/min - 5 ml/min |
|
Flow Velocity |
> 600 cm/hr |
|
Pressure Max. |
1.5 bar [0.15 MPa] (22 psi) |
|
Material |
Polypropylene (PP) |
|
Bed Support & Filter |
Polypropylene (PP) & STS316L Sintered wire mesh filter |
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|
Media |
GE Healthcare Cat.#17-5280-01 / nProtein A 4 FF |
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Ligand |
native Staphylococcal Protein A (S. aureus) |
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Average Particle Size |
90 µm |
|
Type |
native Protein A Affinity |
|
Matrix |
4% cross-linked agarose |
|
Ligand conc. |
6 mg Protein A / ml medium |
|
Ligand coupling method |
Cyanogen bromide activation |
|
Binding Capacity |
> 30 mg/ml hIgG |
|
pH stability |
Working, 3-9
Cleaning, 2-10 |
|
Flow Specification |
< 300 cm/h, 100 kPa, BPX 50/25 column, bed height 15 cm |
|
Exclusion Limit [Mr] [Globular Proteins] |
4 x 106 |
|
Storage Conditions |
4 to 30°C, 20% Ethanol |
|
Estimated Shelflife |
3 years from Manufacture Date |
|
Chemical Stability |
Stable to all commonly used aqueous buffers and 20% ethanol |
|
l CIP : 0.1 N NaOH cleaning is not applicable. 0.1M Glycine(pH3.0) is generally safe for CIP. |
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