recombinant Protein A Pre-packed column
- resin : rProtein A, CaptivATM PriMAP, REPLIGEN
- packing : 5ml, 1set (cat.# DSP-05-07r)
- Accessories included : 1/16" male union, 1ea./set
6mm male union, 1ea./set
2Φx0.5tx1m PTFE tubing(with 6mm union), 1set/set
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BPXTM Ds5-rProtein A : Cat.#DSP-05-07r
BPXTM Ds5-rProtein A are prepacked recombinant Protein A(Repligen BioProcessing's CaptivA™ PriMAB) columns for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants.
CaptivA™ PriMAB
CaptivA™ PriMAB combines a 4% agarose bead with Repligen's new recombinant staphylococcal Protein A ligand (rSPA) ligand. rSPA is an exact amino acid for amino acid copy of the native Protein A sourced from S.aureus, but expressed in E.coli which eliminates concerns associated with the BL2 organism and manufactured with animal free raw materials. CaptivA™ PriMAB provides good all around performance, Protein A leakage, binding capacity and a useful effective working range requiring a residence time of at least 3 minutes (equivalent to a typical running speed of 300cmhr-1).
- Quick, convenient preparative purification of monoclonal antibodies.
- Reliable performance, capacity, flow rates and low leaching
- Products supported by Regulatory Support Files (RSF)
- Well-established protein A-based medium used in many approved MAb processes.
- CIP with 0.1N NaOH is applicable
- High flow rates and high capacities
- Easy & predictable scale-up
- Compatible design for use with syringes, single laboratory pump, or chromatography system
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Column |
BPXTM DSC-101 |
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Column Volume
Bed Volume |
5 ml
5 ml (12Φ x 45 mm) |
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Column Dimensions |
14Φ x 85 mm |
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Flow rate |
1 ml/min - 4 ml/min |
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Flow Velocity |
> 600 cm/hr |
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Pressure Max. |
1.5 bar [0.15 MPa] (22 psi) |
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Material |
Polypropylene (PP) |
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Bed Support & Filter |
Polypropylene (PP) & STS316L Sintered wire mesh filter |
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Media |
Repligen CaptivA™ PriMAB |
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Ligand |
recombinant Staphylococcal Protein A (rSPA), animal free |
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Average Particle Size |
90 µm |
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Type |
rProtein A Affinity |
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Matrix |
4% cross-linked agarose |
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Ligand conc. |
6 mg Potein A / ml medium |
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Ligand coupling method |
Multi-point attachment via Reductive amination |
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Particle Size |
45 µm-165 µm |
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Binding Capacity |
> 40 m/ml hIgG
< 33 mg/ml hIgG under dynamic binding at 3 min residence time |
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pH stability |
Working, 3-10
Cleaning, 2-11 |
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Flow Specification |
< 250 cm/h, 100 kPa, BPX 50/25 column, bed height 15 cm |
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Exclusion Limit [Mr] [Globular Proteins] |
4 x 106 |
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Storage Conditions |
4 to 30°C, 20% Ethanol |
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Estimated Shelflife |
3 years from Manufacture Date |
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Chemical Stability |
Stable to all commonly used aqueous buffers and 20% ethanol1) |
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l CIP : 0.1M Glycine(pH3.0) is generally safe for CIP. Or 0.1 N NaOH cleaning for removing heavy contaminants should be used only for shorter than 30 min. contact (15 min. recommended). |
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