recombinant Protein A Pre-packed column

- resin : rProtein A, CaptivA^TM PriMAP, REPLIGEN

- packing : 10ml, 1set (cat.# DSP-10-07r)

- Accessories included : 1/16" male union, 1ea.
                                  6mm male union, 1ea.
                                  2Φx0.5tx1m PTFE tubing(with 6mm union), 1set
                                  Plug valve, 1ea.; 보너스이벤트
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BPX^TM Ds10-rProtein A : Cat.#DSP-10-07r

BPX^TM Ds10-rProtein A are prepacked recombinant Protein A(Repligen BioProcessing's CaptivA^™ PriMAB) columns for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants.

CaptivA^™ PriMAB

CaptivA^™ PriMAB combines a 4% agarose bead with Repligen's new recombinant staphylococcal Protein A ligand (rSPA) ligand. rSPA is an exact amino acid for amino acid copy of the native Protein A sourced from S.aureus, but expressed in E.coli which eliminates concerns associated with the BL2 organism and manufactured with animal free raw materials. CaptivA^™ PriMAB provides good all around performance, Protein A leakage, binding capacity and a useful effective working range requiring a residence time of at least 3 minutes (equivalent to a typical running speed of 300cmhr^-1).

• Quick, convenient preparative purification of monoclonal antibodies.
• Reliable performance, capacity, flow rates and low leaching
• Products supported by Regulatory Support Files (RSF)
• Well-established protein A-based medium used in many approved MAb processes.
• CIP with 0.1N NaOH is applicable
• High flow rates and high capacities
• Easy & predictable scale-up
• Compatible design for use with syringes, single laboratory pump, or chromatography system

Column	BPXTM DSC-102
Column Volume Bed Volume	10 ml 10 ml (15Φ x 57 mm)
Column Dimensions	17Φ x 102 mm
Flow rate	1 ml/min - 6 ml/min
Flow Velocity	> 600 cm/hr
Pressure Max.	1.5 bar [0.15 MPa] (22 psi)
Material	Polypropylene (PP)
Bed Support & Filter	Polypropylene (PP) & STS316L Sintered wire mesh filter

Media	Repligen CaptivA™ PriMAB
Ligand	recombinant Staphylococcal Protein A (rSPA), animal free
Average Particle Size	90 µm
Type	rProtein A Affinity
Matrix	4% cross-linked agarose
Ligand conc.	6 mg Potein A / ml medium
Ligand coupling method	Multi-point attachment via Reductive amination
Particle Size	45 µm-165 µm
Binding Capacity	> 40 m/ml hIgG < 33 mg/ml hIgG under dynamic binding at 3 min residence time
pH stability	Working, 3-10 Cleaning, 2-11
Flow Specification	< 250 cm/h, 100 kPa, BPX 50/25 column, bed height 15 cm
Exclusion Limit [Mr] [Globular Proteins]	4 x 106
Storage Conditions	4 to 30°C, 20% Ethanol
Estimated Shelflife	3 years from Manufacture Date
Chemical Stability	Stable to all commonly used aqueous buffers and 20% ethanol1)
l CIP : 0.1M Glycine(pH3.0) is generally safe for CIP. Or 0.1 N NaOH cleaning for removing heavy contaminants should be used only for shorter than 30 min. contact (15 min. recommended).