Heparin Sepharose 6 FF, Pre-packed column
- resin : Heparin Sepharose 6 FF, GE Healthcare
- packing : 30ml, 1set (cat.# DSP-30-06)
- Accessories included : 1/16" male union, 1ea.
6mm male union, 1ea.
2Φx0.5tx1m PTFE tubing(with 6mm union), 1set
Plug valve, 1ea.; 보너스이벤트
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BPXTM Ds30-Heparin : Cat.#DSP-30-06
BPXTM Ds30-Heparin are prepacked Heparin Sepharose 6 Fast Flow columns for preparative purification of proteins with affinity for heparin such as DNA-binding proteins, coagulation factors, lipoproteins, and protein synthesis factors. This medium is extensively used for industrial purification of several marketed plasma proteins.
- Quick, convenient preparative purification of antithrombin III, coagulation factors and other plasma proteins
- Coupling chemistry gives chemically-stable ligand attachment. Stability of medium solely dictated by heparin ligand.
- High flow rates and high capacities
- Easy & predictable scale-up
- Compatible design for use with syringes, single laboratory pump, or chromatography system
The column provides fast, preparative separations of proteins and other biomolecules. Immobilized heparin has two main modes of interaction with proteins. It can operate as an affinity ligand with affinity for a wide range of biomolecules, including coagulation factors and other plasma proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. Heparin also functions as a high-capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases.
Heparin Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified sodium heparin. Immobilization is performed using chemically stable and oriented coupling giving high binding capacity. The chemical stability of the media is solely dictated by the heparin ligand.
This medium allows for fast and reliable separations of biomolecules with an affinity for heparin, including antithrombin III, coagulation factors and other plasma proteins. Additional applications include DNA binding proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors.
|
Column |
BPXTM DSC-301 |
|
Column Volume
Bed Volume |
10~30 ml
30 ml (22Φ x 79 mm) |
|
Column Dimensions |
24Φ x 133 mm |
|
Flow rate |
2 ml/min - 20 ml/min |
|
Flow Velocity |
> 600 cm/hr |
|
Pressure Max. |
1.5 bar [0.15 MPa] (22 psi) |
|
Material |
Polypropylene (PP) |
|
Bed Support & Filter |
Polypropylene (PP) & STS316L Sintered wire mesh filter |
|
|
|
Media |
GEH Cat.#17-0998-01 / Heparin Sepharose 6 FF |
|
Ligand |
Porcine heparin |
|
Average Particle Size |
90 µm |
|
Type |
Group specific Affinity |
|
Matrix |
6% cross-linked agarose |
|
Particle Size |
45 µm-165 µm |
|
Ligand conc. |
4 mg heparin / ml medium |
|
Ligand coupling method |
Reductive Amination |
|
pH stability |
Working, 4-12
Cleaning, 4-13 |
|
Flow Specification |
200-400 cm/h, 100 kPa, BPX 50/25 column, bed height 15 cm |
|
Exclusion Limit [Mr] [Globular Proteins] |
4 x 106 |
|
Storage Conditions |
4 to 30°C, 20% Ethanol |
|
Estimated Shelflife |
5 years from Manufacture Date |
|
Chemical Stability |
Stable in commonly used aqueous buffers - 0.1 M NaOH, 0.05 M sodium acetate, 8 M urea, 6 M guanidine hydrochloride, non-ionic detergents, 10% glycerol |
|
l CIP : 0.1 N NaOH should be used only for cleaning purposes. | |
