[ÀÌÀü»óÇ°] [¸ñ·Ïº¸±â] [´ÙÀ½»óÇ°]
Eco52 I (100U/vl Cat.#1110), "Àü¿ë + ¨Î" buffer
»óÇ°°¡ 55,000¿ø(ºÎ°¡¼¼Æ÷ÇÔ)
±¸¸Å¼ö·®

°³
Á¦Á¶»ç ºö½º¹ÙÀÌ¿À
¿ø»êÁö Çѱ¹
»óÇ°ÄÚµå 1110
»óÇ°¿É¼Ç
Àç°í¼ö·® 2°³




  Store at -20¡É. For Research Use Only!

CpG

Eco52  I

BseX3 I, BstZ I, Eag I, EclX I

5' ¡¦ C G G C C G ¡¦ 3'
3' ¡¦ G C C G G C ¡¦ 5'

#1110

100 units

#1110L

500 units

Supplied with : 10X Reaction Buffer 'Eco52 I', 10X BSA


Concentration :            units/§¡


Lot # :                


Expiration date :                


Source : Escherichia  coli  RFL52


Compatible ends (5'¡¦GGCC)

Aco I, BseX3 I, Bsp120 I, BstZ I, CciN I, Cfr I, Eae I, Eag I, EclX I, Gdi II, Not I, PspOM I, Xma III


Isoschizomers : BseX3 I, BstZ I, Eag I, EclX I


Methylation Sensitivity

: Not sensitive to dam and dcm methylation

: Blocked by overlapping CpG methylation


Unit definition : One Unit is defined as the amount of Eco52 I required to completely digest 1 §¶ of ¥ë DNA in one hour at 37¡É in 50 §¡ assay mixture.


Typical Reaction Conditions : Add 5 §¡ of 10X Reaction Buffer 'Eco52 I'[final conc.: 10 mM Tris-HCl (pH 8.9 @ 37¡É), 3 mM MgCl2, 100 mM NaCl], 1~2 §¡ of DNA(0.5~1.0 §¶/§¡), 5 §¡ of 10X BSA(final conc.: 100 §¶/§¢), 1 §¡ of Eco52 I and 37~38 §¡ of sterile water in 50 §¡ reaction mixture. Incubate at 37¡É.


Heat Inactivation : 65¡É for 20 min.


Activity in BeamsBio¢â Buffer System

Rxn Buffer

A

B

C

D

'Eco52 I'

Activity(%)

<10

<10

<10

<10

75~100


Storage Conditions : 10 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 200 §¶/§¢ BSA, and 50% glycerol. Store at -20¡É.


No. of Cleavage Sites

¥ë

Ad2

pUC18

¥õX174

pBR322

M13mp18

SV40

2

19

0

0

1

0

0


Quality control

Overdigestion: The same band pattern as a digestion in one unit of Eco52 I for one hour was showing after incubation of 1 §¶ of ¥ë DNA with         units of Eco52 I for 16 hr in 50 §¡ of reaction mixture at 37¡É as determined by agarose gel electrophoresis.

Ligation-Recut: After a 10-fold digestion for one hour,        % of ¥ë DNA fragments were ligated with T4 DNA ligase at 16¡É and        % of the ligated fragments could be recut with Eco52 I.


Notes


References

Janulaitis, A., Stakenas, P.S., Petrusyte, M.P., Bitinaite, J.B., Klimasauskas, S.I., Butkus, V.V. Mol. Biol. (Mosk) 18: 115-129 (1983)


Beams Biotechnology Co., Ltd.


1. 9¸¸¿ø ÀÌÇÏ(VAT º°µµ ±Ý¾×) ÁÖ¹® ½Ã, ³Ãµ¿ È­¹° ºñ¿ëÀº 11,000¿ø ÀÔ´Ï´Ù.  
2. ¿ù 09:00 ~ ¸ñ¿äÀÏ 15:00»çÀÌ ³Ãµ¿¹è¼ÛÁ¦Ç° ÁÖ¹®Àº Á¤»ó ¹è¼ÛÀÌ ÀÌ·ç¾îÁö³ª, ¸ñ¿äÀÏ 15:00 ÀÌÈÄ ÁÖ¹®Àº ¹è¼Û»ç°í ¿¹¹æÀ» À§ÇØ Â÷ÁÖ ¿ù¿äÀÏ¿¡ ¹è¼ÛÀÌ ÀÌ·ç¾îÁý´Ï´Ù.  ´Ü, °í°´ÀÌ ¹°Ç°Àμö¸¦ Ã¥ÀÓÁö´Â °æ¿ì¿¡´Â ±Ý¿äÀÏ¿¡µµ ¹è¼ÛÇÒ ¼ö ÀÖ½À´Ï´Ù.
3. ´ç»ç¿¡¼­´Â ³Ãµ¿È­¹°ÀÇ Ç°ÁúÀÌ ¹ß¼Û ÈÄ ÃÖ´ë 30½Ã°£±îÁö À¯ÁöµÉ ¼ö ÀÖµµ·Ï µå¶óÀ̾ÆÀ̽º¿Í ¾óÀ½À» »ç¿ëÇÏ¿© ³Ãµ¿Æ÷ÀåÀ» ½Ç½ÃÇÕ´Ï´Ù.
4. °í°´´Ô²²¼­´Â Àμö°ÅºÎ³ª ÀμöÀÚ ºÎÁÖÀÇ·Î ÀÎÇÑ ¸Á½Ç, ³Ãµ¿È­¹°ÀÇ µµÂøÀÌÈÄ ¹æÄ¡ µî °í°´´ÔÀÇ °ú½Ç¿¡ ÀÇÇÑ Ç°Áú º¯ÁúÀÌ ¹ß»ýÇÏÁö ¾Êµµ·Ï À¯ÀÇÇÏ¼Å¾ß ÇÕ´Ï´Ù. 
5. ¹è¼ÛÀÌ ÀÌ·ç¾îÁö¸é °í°´´Ô²² Åùè»ç¿Í ¿î¼ÛÀå¹øÈ£°¡ À̸ÞÀÏ·Î ÅëÁöµË´Ï´Ù.  °í°´´Ô²²¼­´Â ¿î¼ÛÀå¹øÈ£·Î Åùè°úÁ¤À» ÃßÀûÇÒ ¼ö ÀÖ½À´Ï´Ù.



¢ßºö½º¹ÙÀÌ¿À¿¡¼­ ÆǸÅÇÑ ¹ÙÀÌ¿À¼ÒÀç Á¦Ç°ÀÇ Ç°Áú ºÒ¸¸¿¡ ´ëÇÏ¿© °ø±ÞÀÚ¸¦ ÅëÇÏ¿© ´ç»ç¿Í ¹Ì¸® ÇùÀÇ°¡ ¿Ï·áµÈ °æ¿ì º°µµÀÇ ºñ¿ë ºÎ´ã¾øÀÌ Á¦Ç°À» ±³È¯ÇÏ¿© µå¸³´Ï´Ù. µû¶ó¼­, Ç°Áú ºÒ¸¸¿¡ ´ëÇÏ¿© ¹Ì¸® ÇùÀÇ°¡ ÀÌ·ç¾îÁö±â Àü±îÁö´Â ¹°Ç°À» ÀÓÀÇ·Î ¹Ý¼ÛÇÏ¿©¼­´Â ¾ÈµÇ¸ç, ÀÌ °æ¿ì ¹ß»ýÇÏ´Â ¸ðµç ºñ¿ëÀº °í°´ÀÌ ºÎ´ãÇÏ¿©¾ß ÇÕ´Ï´Ù. ¹ÙÀÌ¿À ¼ÒÀç Á¦Ç°Àº ¿î¼Û ½Ã Ç°ÁúÀÌ º¯µ¿µÉ ¼ÒÁö°¡ Å©±â ¶§¹®¿¡ Á¦Ç° Ç°Áú ¹®Á¦´Â ´ë°³ ¿î¼Û µµÁß¿¡ ¹ß»ýÇÕ´Ï´Ù´ç»ç¿¡¼­´Â ¹Ý¼ÛµÈ ¹°Ç°À» °Ë»ç ÈÄ °Ë»ç °á°ú¿¡ »ó°ü¾øÀÌ Áï½Ã Æó±âÇÕ´Ï´Ù.

A return authorization is required before shipping.  Please do not return any product without first contacting Beams Biotechnology or distributor for a return authorization.  All return authorizations must be issued directly from Beams Biotechnology.  In the case of a purchasing error, it is our policy to deny return of product.  Due to the temperature sensitivity of the products, we are unable to resell returned goods, as we are not assured of the quality.






 
             (ÁÖ)ºö½º¹ÙÀÌ¿À 13210 °æ±âµµ ¼º³²½Ã Áß¿ø±¸ »ç±â¸·°ñ·Î 52 (¼±ÅýÃƼ2) B104È£ ÀüÈ­:031-737-4970 Æѽº:031-737-4972
»ç¾÷ÀÚµî·Ï¹øÈ£:129-81-99161 Åë½ÅÆǸž÷½Å°í¹øÈ£:°æ±â ¼º³² Á¦2007-006È£ ´ëÇ¥ÀÌ»ç:±èö °³ÀÎÁ¤º¸º¸È£Ã¥ÀÓÀÚ:±èö
Copyright¨Ï2021 (ÁÖ)ºö½º¹ÙÀÌ¿À All Rights Reserved.