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Mung Bean Nuclease / 1.5KU / Cat.#3006
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  Mung Bean Nuclease

    

#3006

 

1,500 units

#3006L

 

7,500 units

 

 

DESCRIPTION 

Mung Bean Nuclease degrades single-stranded nucleic acids to yield 5'-phosphoryl mono- or oligonucleotides.  Double-stranded DNA, RNA and DNA:RNA hybrids are relatively resistant to the enzyme.  If very large amount of enzyme are used it also degrades double-stranded DNA from both ends.  Unlike S1 nuclease, this enzyme will not cleave the DNA strand opposite a nick in a duplex. 

 

 

SOURCE 

Mung bean sprouts. 

 

 

APPLICATIONS 

¡Ü Removal of 3' and 5' extensions from DNA or RNA 

termini. 

¡Ü Transcript mapping. 

¡Ü Opening up hairpin loops formed during synthesis of 

double-stranded cDNA. 

¡Ü Excision of gene coding sequences from genomic 

  DNA. 

 

 

 

 

UNIT DEFINITION 

One unit is defined as the amount of enzyme required to produce 1¥ìg of acid-soluble nucleotides 1 minutes at 37¡É. 

 

 

STANDARD UNIT ASSAY CONDITIONS 

30mM sodium acetate (pH4.6), 50mM NaCl, 1mM ZnCl2, 0.5mg/ml denatured calf thymus DNA and 5%(v/v) glycerol. 

 

 

CONCENTRATION: 50-100 units/ul. 

 

 

10X REACTION BUFFER 

300mM sodium acetate(pH5.0), 500mM NaCl, 10mM ZnCl2. 

 

 

STORAGE CONDITIONS 

10mM sodium acetate (pH5.0), 0.1mM zinc acetate, 1mM cysteine, 0.001% Triton X-100, and 50% glycerol.  Store at -20¡É. 

 

 

REFERENCES 

1. Kowalski, D. et al. (1976) Biochemistry 15, 4457. 

2. Green, M.R. and Roeder, R.G. (1980) Cell 22, 231. 

3. Gubler, U. (1987) Methods Enzymol. 152, 330. 

 

 


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