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Phenyl Sepharose 6 FF (hs), Prepacked column, 20ml
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Phenyl Sepharose 6 FF(high), Pre-packed column

- resin : Phenyl Sepharose 6 FF (high substitution) GEH Cat.17-0973-01

- packing : 20ml, 1set (cat.# DSP-20-03)

- Accessories included : 1/16" male union, 1ea.
                                   6mm male union, 1ea.
                                   2¥Õx0.5tx1m PTFE tubing(with 6mm union), 1set
 
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BPXTM Ds20-Phenyl :  Cat.#DSP-20-03

BPXTM Ds20-Phenyl are prepacked Phenyl Sepharose 6 Fast Flow(high substitution) columns for hydrophobic interaction chromatography (HIC). The columns are used for small-scale protein purification as well as for screening of binding and elutions conditions.

  • Convenient and easy small-scale protein purifications.
  • Phenyl Sepharose 6 Fast Flow is a well-established, standard aromatic HIC medium for capture and intermediate purification requiring low to medium hydrophobicity.
  • Flexible design for use with syringes, single laboratory pump, or chromatography system such as ÄKTA design.
  • High flow rates and high capacities.
  • Easy & predictable scale-up
  • Compatible design for use with syringes, single laboratory pump, or chromatography system

Column

BPXTM DSC-301

Column Volume

Bed Volume

10~30 ml

20 ml (22¥Õ x 53 mm)

Column Dimensions

24¥Õ x 133 mm

Flow rate

2 ml/min - 20 ml/min

Flow Velocity

> 600 cm/hr

Pressure Max.

1.5 bar [0.15 MPa] (22 psi)

Material

Polypropylene (PP)

Bed Support & Filter

Polypropylene (PP) & STS316L Sintered wire mesh filter

Media

GEH Cat.#17-0973-01 / Phenyl Sepharose 6 FF (High substitution)

Ligand

Spacer arm

Phenyl

2-hydroxypropyl

Average Particle Size

90 µm

Type

Hydrophobic Interaction Chromatography

Matrix

6% cross-linked agarose

Particle Size

45 µm-165 µm

Degree of substitution

approx. 40 ¥ìmol /ml medium

pH stability

Working, 3-13

Cleaning, 2-14

Flow Specification

200-400 cm/h, 100 kPa, BPX 50/50 column, bed height 25 cm

Storage Conditions

4 to 30¡ÆC, 20% Ethanol

Estimated Shelflife

5 years from Manufacture Date

Chemical Stability

Stable in commonly used aqueous buffers - 1 M NaOH, 3 M Ammonium sulphate, 30% isopropanol, 70% ethanol, 10% ethylene glycol, 0.5% SDS, 6 M guanidine-hydrochloride, 8 M Urea

l  CIP : 1 N NaOH should be used only for cleaning purposes.

 







 
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