[ÀÌÀü»óÇ°] [¸ñ·Ïº¸±â] [´ÙÀ½»óÇ°]
Ni-IMAC, Workbead40 IDA(high)/BIOWORKS, Prepacked column, 10ml
»óÇ°°¡ 170,500¿ø(ºÎ°¡¼¼Æ÷ÇÔ)
±¸¸Å¼ö·®

°³
Á¦Á¶»ç ºö½º¹ÙÀÌ¿À
¿ø»êÁö Çѱ¹
»óÇ°ÄÚµå DSP-10-05



Ni-IMAC IDA(high) Workbead40, Pre-packed column

- resin : Workbead40 IDA(high) Ni-loaded, BioWorks

- packing : 10ml, 1set (cat.# DSP-10-05)

- Accessories included : 1/16" male union, 1ea.
                                  6mm male union, 1ea.
                                  2¥Õx0.5tx1m PTFE tubing(with 6mm union), 1set

-----------------------------------------------------------------------------

BPXTM Ds10-Ni IDA : Cat.#DSP-10-05

BPXTM Ds10-Ni IDA are prepacked WorkBeads 40 IDA (high) columns pre-loaded with Ni+2, a high throughput agarose media for Immobilized Metal Affinity Chromatography (IMAC) designed for capture or intermediate purification steps.

  • Choice of IMAC Chemistry to Fit a Large Variety of Proteins
  • High Precision and High Flow Characteristics by using 40¥ìm
  • Made from Agarose, Well Established and Well-known in the Biotechnology Industry
  • The well-known Iminodiacetic acid (IDA) used for chelator and preloaded with Ni+2.
  • High flow rates and high capacities
  • Easy & predictable scale-up
  • Compatible design for use with syringes, single laboratory pump, or chromatography system

Metal Ions and Loading

The following metal ions have been used most fre-quently for IMAC: Zn2+, Cu2+, Ni2+, Fe3+, Co2+, Ca2+ and Al3+ but in principle all metal ions known to interact with proteins can be used. It should be noted that the total capacity for the dif-ferent gels are specified only for Cu2+ and will vary slightly for other metal ions. A 50 mM solution of a suitable metal is prepared in distilled water. Some care has to be taken when selecting the loading buffer. The concentration of metal ion will be rather high when absorbed to the gel and precipitation may occur. Normally a 0.1 M sodium acetate buffer pH 5.5 can be used. The metal salt solution is added through a sample loop by repeated injection until the gel is fully loaded.

Adsorption and Desorption Conditions

These conditions vary of course with the separation problem. Adsorption solvents are normally aqueous but organic solvents in low concentration can also be used. Depending on the nature of the chelator both electrostatic forces and hydrophobic interaction may be involved and care has to be taken with the ionic strength of the buffer. Buffers containing groups with affinity for the metal ion should be avoided (e.g. imidazole.)  Protein desorption is either done by change of the pH or by competition for the metal binding sites between the protein and another compound like ammonium salts or imidazole buffer.

Removal of Metal Ions

Many metal ions undergo redox reactions and this may cause deviations during storage of the gel. If the gel is not going to be used for a long time, the removal of metal ions is strongly recommended. This is easily done with 0.1 M solution of ethylenediamine tetra acetic acid (EDTA) either through repeated injec-tion via e.g. a sample loop or directly through the pump.

Column

BPXTM DSC-102

Column Volume

Bed Volume

10 ml

10 ml (15¥Õ x 57 mm)

Column Dimensions

17¥Õ x 102 mm

Flow rate

1 ml/min - 10 ml/min

Flow Velocity

> 600 cm/hr

Pressure Max.

1.5 bar [0.15 MPa] (22 psi)

Material

Polypropylene (PP)

Bed Support & Filter

Polypropylene (PP) & STS316L Sintered wire mesh filter


Media

Bio-Works, WorkBeads 40 IDA (high), pre-loaded with Ni2+

Chelating group

Ligand

Iminodiacetic acid (IDA)

Ni+2

Average Particle Size

40 µm

Type

 Immobilized Metal Affinity Chromatography (IMAC)

Matrix

7.4-7.8% cross-linked agarose

Particle Size

32 µm-60 µm

Metal Ion Capacity

 40-50 ¥ìeqv Cu2+ / mL medium

Ligand coupling method

  the Bromohydrin method

pH stability

Working, 2-13

Cleaning, 2-14

Flow Specification

200-400 cm/h, 200 kPa, BPX 50/25 column, bed height 15 cm

Exclusion Limit [Mr] [Globular Proteins]

4 x 106

Storage Conditions

4 to 30¡ÆC, 20% Ethanol

Estimated Shelflife

5 years from Manufacture Date

Chemical Stability

100% methanol, 100% ethanol, 8 M urea, 6 M guanidine hydrochloride, 30% acetonitrile, 70% formic acid, 30% trifluoroacetic acid

l  CIP : 0.5 N NaOH and acetic acid should be used only for cleaning purposes.








 
             (ÁÖ)ºö½º¹ÙÀÌ¿À 13210 °æ±âµµ ¼º³²½Ã Áß¿ø±¸ »ç±â¸·°ñ·Î 52 (¼±ÅýÃƼ2) B104È£ ÀüÈ­:031-737-4970 Æѽº:031-737-4972
»ç¾÷ÀÚµî·Ï¹øÈ£:129-81-99161 Åë½ÅÆǸž÷½Å°í¹øÈ£:°æ±â ¼º³² Á¦2007-006È£ ´ëÇ¥ÀÌ»ç:±èö °³ÀÎÁ¤º¸º¸È£Ã¥ÀÓÀÚ:±èö
Copyright¨Ï2021 (ÁÖ)ºö½º¹ÙÀÌ¿À All Rights Reserved.