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native Protein A (GEH), Pre-packed column, 5ml
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Native Protein A Pre-packed column

- resin : nProtein A Sepharose 4 FF, GE Healthcare

- packing : 5ml, 1set (cat.# DSP-05-07n)

- Accessories included : 1/16" male union, 1ea.
                                   6mm male union, 1ea.
                                   2¥Õx0.5tx1m PTFE tubing(with 6mm union), 1set

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BPXTM Ds5-nProtein A are prepacked native Protein A coupled to Sepharose 4 Fast Flow for the purification of monoclonal and polyclonal antibodies at both laboratory and process scale.

nProtein A Sepharose 4 Fast Flow is native protein A coupled to the well established Sepharose 4 Fast Flow base matrix. The native protein A ligand is produced by fermenting a selected strain of Staphylococcus aureus. The purified protein is coupled to the cross-linked 4% agarose base matrix by the cyanogen bromide technique, giving a highly stable medium with minimal non-specific adsorption. nProtein A Sepharose 4 Fast Flow is manufactured without using animal-derived components.

nProtein A Sepharose 4 Fast Flow has nearly twice the total IgG binding capacity of Protein A Sepharose CL-4B, and is suitable for recovery and purification of monoclonal antibodies from cell culture at both laboratory and process scale. nProtein A Sepharose 4 Fast Flow was developed and tested in co-operation with leading manufacturers of purified monoclonal antibody products, and is used in routine commercial production.

  • Quick, convenient preparative purification of monoclonal antibodies.
  • Free from animal-derived components.
  • Well-established protein A-based medium used in many approved MAb processes.
  • Recombinant protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
  • High flow rates and high capacities
  • Easy & predictable scale-up
  • Compatible design for use with syringes, single laboratory pump, or chromatography system

Column

BPXTM DSC-101

Column Volume

Bed Volume

5 ml

5 ml (12¥Õ x 45 mm)

Column Dimensions

14¥Õ x 85 mm

Flow rate

1 ml/min - 5 ml/min

Flow Velocity

> 600 cm/hr

Pressure Max.

1.5 bar [0.15 MPa] (22 psi)

Material

Polypropylene (PP)

Bed Support & Filter

Polypropylene (PP) & STS316L Sintered wire mesh filter


Media

GE Healthcare Cat.#17-5280-01 / nProtein A 4 FF

Ligand

native Staphylococcal Protein A (S. aureus)

Average Particle Size

90 µm

Type

native Protein A Affinity

Matrix

4% cross-linked agarose

Ligand conc.

6 mg Protein A / ml medium

Ligand coupling method

Cyanogen bromide activation

Binding Capacity

> 30 mg/ml hIgG

pH stability

Working, 3-9

Cleaning, 2-10

Flow Specification

< 300 cm/h, 100 kPa, BPX 50/25 column, bed height 15 cm

Exclusion Limit [Mr] [Globular Proteins]

4 x 106

Storage Conditions

4 to 30¡ÆC, 20% Ethanol

Estimated Shelflife

3 years from Manufacture Date

Chemical Stability

Stable to all commonly used aqueous buffers and 20% ethanol

l  CIP : 0.1 N NaOH cleaning is not applicable. 0.1M Glycine(pH3.0) is generally safe for CIP.







 
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