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recombinant Protein A, Pre-packed column, 10ml, Captiva(TM) PRIMAB
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 recombinant Protein A Pre-packed column

- resin : rProtein A, CaptivA
TM PriMAP, REPLIGEN

- packing : 10ml, 1set (cat.# DSP-10-07r)

- Accessories included : 1/16" male union, 1ea.
                                  6mm male union, 1ea.
                                  2¥Õx0.5tx1m PTFE tubing(with 6mm union), 1set
                                  Plug valve, 1ea.; º¸³Ê½ºÀ̺¥Æ®
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BPXTM Ds10-rProtein A : Cat.#DSP-10-07r

BPXTM Ds10-rProtein A are prepacked recombinant Protein A(Repligen BioProcessing's CaptivA¢â PriMAB) columns for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants.

CaptivA¢â PriMAB

CaptivA¢â PriMAB combines a 4% agarose bead with Repligen's new recombinant staphylococcal Protein A ligand (rSPA) ligand. rSPA is an exact amino acid for amino acid copy of the native Protein A sourced from S.aureus, but expressed in E.coli which eliminates concerns associated with the BL2 organism and manufactured with animal free raw materials. CaptivA¢â PriMAB provides good all around performance, Protein A leakage, binding capacity and a useful effective working range requiring a residence time of at least 3 minutes (equivalent to a typical running speed of 300cmhr-1).

  • Quick, convenient preparative purification of monoclonal antibodies.
  • Reliable performance, capacity, flow rates and low leaching
  • Products supported by Regulatory Support Files (RSF)
  • Well-established protein A-based medium used in many approved MAb processes.
  • CIP with 0.1N NaOH is applicable
  • High flow rates and high capacities
  • Easy & predictable scale-up
  • Compatible design for use with syringes, single laboratory pump, or chromatography system

Column

BPXTM DSC-102

Column Volume

Bed Volume

10 ml

10 ml (15¥Õ x 57 mm)

Column Dimensions

17¥Õ x 102 mm

Flow rate

1 ml/min - 6 ml/min

Flow Velocity

> 600 cm/hr

Pressure Max.

1.5 bar [0.15 MPa] (22 psi)

Material

Polypropylene (PP)

Bed Support & Filter

Polypropylene (PP) & STS316L Sintered wire mesh filter

Media

Repligen CaptivA¢â PriMAB

Ligand

recombinant Staphylococcal Protein A (rSPA), animal free

Average Particle Size

90 µm

Type

rProtein A Affinity

Matrix

4% cross-linked agarose

Ligand conc.

6 mg Potein A / ml medium

Ligand coupling method

Multi-point attachment via Reductive amination

Particle Size

45 µm-165 µm

Binding Capacity

> 40 m/ml hIgG

< 33 mg/ml hIgG under dynamic binding at 3 min residence time

pH stability

Working, 3-10

Cleaning, 2-11

Flow Specification

< 250 cm/h, 100 kPa, BPX 50/25 column, bed height 15 cm

Exclusion Limit [Mr] [Globular Proteins]

4 x 106

Storage Conditions

4 to 30¡ÆC, 20% Ethanol

Estimated Shelflife

3 years from Manufacture Date

Chemical Stability

Stable to all commonly used aqueous buffers and 20% ethanol1)

l CIP : 0.1M Glycine(pH3.0) is generally safe for CIP. Or 0.1 N NaOH cleaning for removing heavy contaminants should be used only for shorter than 30 min. contact (15 min. recommended).







 
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