[ÀÌÀü»óÇ°] [¸ñ·Ïº¸±â] [´ÙÀ½»óÇ°]
Heparin Sepharose 6 FF, Pre-packed column, 30ml
»óÇ°°¡ 693,000¿ø(ºÎ°¡¼¼Æ÷ÇÔ)
±¸¸Å¼ö·®

°³
Á¦Á¶»ç BEAMSBIO
»óÇ°ÄÚµå DSP-30-06
»óÇ°¿É¼Ç



Heparin Sepharose 6 FF, Pre-packed column

- resin : Heparin Sepharose 6 FF, GE Healthcare

- packing : 30ml, 1set (cat.# DSP-30-06)

- Accessories included : 1/16" male union, 1ea.
                                  6mm male union, 1ea.
                                  2¥Õx0.5tx1m PTFE tubing(with 6mm union), 1set
                                  Plug valve, 1ea.; º¸³Ê½ºÀ̺¥Æ®
----------------------------------------------------------------------------------------------------------



BPXTM Ds30-Heparin : Cat.#DSP-30-06

BPXTM Ds30-Heparin are prepacked Heparin Sepharose 6 Fast Flow columns for preparative purification of proteins with affinity for heparin such as DNA-binding proteins, coagulation factors, lipoproteins, and protein synthesis factors. This medium is extensively used for industrial purification of several marketed plasma proteins.

  • Quick, convenient preparative purification of antithrombin III, coagulation factors and other plasma proteins
  • Coupling chemistry gives chemically-stable ligand attachment. Stability of medium solely dictated by heparin ligand.
  • High flow rates and high capacities
  • Easy & predictable scale-up
  • Compatible design for use with syringes, single laboratory pump, or chromatography system

The column provides fast, preparative separations of proteins and other biomolecules. Immobilized heparin has two main modes of interaction with proteins. It can operate as an affinity ligand with affinity for a wide range of biomolecules, including coagulation factors and other plasma proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. Heparin also functions as a high-capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases.

Heparin Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified sodium heparin. Immobilization is performed using chemically stable and oriented coupling giving high binding capacity. The chemical stability of the media is solely dictated by the heparin ligand.

This medium allows for fast and reliable separations of biomolecules with an affinity for heparin, including antithrombin III, coagulation factors and other plasma proteins. Additional applications include DNA binding proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors.

Column

BPXTM DSC-301

Column Volume

Bed Volume

10~30 ml

30 ml (22¥Õ x 79 mm)

Column Dimensions

24¥Õ x 133 mm

Flow rate

2 ml/min - 20 ml/min

Flow Velocity

> 600 cm/hr

Pressure Max.

1.5 bar [0.15 MPa] (22 psi)

Material

Polypropylene (PP)

Bed Support & Filter

Polypropylene (PP) & STS316L Sintered wire mesh filter

Media

GEH Cat.#17-0998-01 / Heparin Sepharose 6 FF

Ligand

Porcine heparin

Average Particle Size

90 µm

Type

Group specific Affinity

Matrix

6% cross-linked agarose

Particle Size

45 µm-165 µm

Ligand conc.

4 mg heparin / ml medium

Ligand coupling method

Reductive Amination

pH stability

Working, 4-12

Cleaning, 4-13

Flow Specification

200-400 cm/h, 100 kPa, BPX 50/25 column, bed height 15 cm

Exclusion Limit [Mr] [Globular Proteins]

4 x 106

Storage Conditions

4 to 30¡ÆC, 20% Ethanol

Estimated Shelflife

5 years from Manufacture Date

Chemical Stability

Stable in commonly used aqueous buffers - 0.1 M NaOH, 0.05 M sodium acetate, 8 M urea, 6 M guanidine hydrochloride, non-ionic detergents, 10% glycerol

l CIP : 0.1 N NaOH should be used only for cleaning purposes.







 
             (ÁÖ)ºö½º¹ÙÀÌ¿À 13210 °æ±âµµ ¼º³²½Ã Áß¿ø±¸ »ç±â¸·°ñ·Î 52 (¼±ÅýÃƼ2) B104È£ ÀüÈ­:031-737-4970 Æѽº:031-737-4972
»ç¾÷ÀÚµî·Ï¹øÈ£:129-81-99161 Åë½ÅÆǸž÷½Å°í¹øÈ£:°æ±â ¼º³² Á¦2007-006È£ ´ëÇ¥ÀÌ»ç:±èö °³ÀÎÁ¤º¸º¸È£Ã¥ÀÓÀÚ:±èö
Copyright¨Ï2021 (ÁÖ)ºö½º¹ÙÀÌ¿À All Rights Reserved.