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▶ Preparation of Inoculum, Main Culture in 300ℓ Pilot Fermentor
▶ Batch Culture, Fed-Batch or Continuous Culture
▶ pH Control, pO2 Control or DO Stat Operation, Pressurized Culture upto 1.5atm
▶ Harvest of Wet Cells by Centrifugation or Microfiltration
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① Intracellular Protein
- Recovery of Wet Cells
- Gaulin Press, Ultrasonic Dismembrator, Dyno-Mil or Chemical Lysis
- Removal of Cell Debris and Nucleic Acids, Fractionation and Dialysis
② Extracellular Protein
- Recovery of Cell Broth by centrifugation
- Ultrafiltration of Broth : Lab-scale or Process-scale
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▶ Ion Exchange, Affinity, Gel Filtration, Ultrafiltration, Dialysis and Concentration
▶ PAGE or SDS-PAGE Gel Electrophoresis, Quality Control Assay
▶ Lab-scale Chromatography with FPLC System
▶ Process-scale Chromatography with C3 System
▶ Removal of Endotoxin. Freeze Drying
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